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Liu Wen is a woman of many firsts. She's the first East Asian model to work for Victoria's Secret, first Chinese woman to grace American Vogue's cover, and first Asian to ever make Forbes' list of highest-paid supermodels.

While Liu success can be attributed to the fashion industry's growing focus on Chinese consumers, she also works insanely hard for it.

The model, now based between New York and Beijing, told Business Insider how she did it. Scroll on to learn more.

Meet Liu Wen: The first East Asian model to walk the Victoria's Secret runway, first Chinese model to grace American Vogue's cover, and first Asian model to ever make Forbes' annual list of highest-paid models.

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Source: , Vogue

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European Commission. Commission Regulation (EU) No 1004/2014. Official Journal of the European Union, 2014. Available online: http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32014R1004rid=1 .

http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32014R1004rid=1

CDC. Resorcinol. NIOSH Pocket Guide to Chemical Hazards, 2015. Available online: http://www.cdc.gov/niosh/npg/npgd0543.html.

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IARC. Monographs on the evaluation of carcinogenic risks to humans. IARC PREss, volume 82, 2002. Available online: http://monographs.iarc.fr/ENG/Monographs/vol82/mono82.pdf .

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Kang C, et al. Polybrominated diphenyl ethers and synthetic musk in umbilical cord serum, maternal serum, and breast milk from Seoul, South Korea. Chemosphere, vol. 80, pp 116-122, 2010.

Zhang X, Jing Y, Ma L, Zhou J, Fang X, Zhang X, Yu Y. Occurrence and transport of synthetic musks in paired maternal blood, umbilical cord, and breast milk. International Journal of Hygiene and Environmental Health, vol. 218, pp 99-106, 2015.

Reiner J, Wong C, Arcano K, Kannan K. Synthetic musk fragrances in human milk from the United States. Environ. Sci. Technol., vol. 41, pp 3815-3820, 2007.

Kannan K, Reiner J, Yun S, Perrotta E, Tao L, Johnson-Restrepo, Rodan B. Polycyclic musk compounds in higher trophic level aquatic organisms and humans from the United States. Chemosphere, vol. 61, pp 693-700, 2005.

Petersen K, Tollefsen K. Assessing combined toxicity of estrogen receptor agonists in a primary of culture of rainbow trout ( Oncorhynchus mykiss ) hepatocytes. Aquatic toxicology, vol. 101, pp 186-195, 2011.

Witorsch R, Thomas J. Personal care products and endocrine disruption: a critical review of the literature. Critical Reviews in Toxicology, vol. 40, no. S3, pp 1-30, 2010.

Schreurs R, Sonneveld E, Jansen J, Seinen W, van der Burg B. Interaction of polycyclic musks and UV filters with the estrogen receptor (ER), androgen receptor (AR), and progesterone receptor (PR) in reporter gene bioassays. Toxicological Sciences, vol. 83, pp 264-272, 2005.

Fig. S1.

Size distribution of plasma DNA fragments carrying fetal-specific alleles (blue) and alleles shared by the fetus and the mother (red).

Fig. S2.

Frequency plot of the size difference between plasma DNA fragments carrying fetal-specific alleles and alleles shared by the fetus and the mother at SNPs where the mother was homozygous and the fetus was heterozygous for the third trimester pregnancy case. The red dotted line indicates the size difference observed in 90% of the SNPs. () Third trimester case. () The case carrying a fetus with cardiofaciocutaneous syndrome.

Fifty-six single-nucleotide variants were found to be present in the umbilical cord blood cells and absent in the parents. These 56 single-nucleotide variants were thus deemed de novo mutations possessed by the fetus. Deep genome-wide sequencing is needed for the screening of fetal de novo mutations from maternal plasma. At this level of sequencing, a vast number of sequencing errors would be generated and hinder the sensitive and specific detection of true fetal de novo mutations. To tackle the challenge, we designed a bioinformatics protocol that used a series of steps to filter out the false-positive mutations ( Fig. 2 ). The bioinformatics protocol made use of steps that identified high-quality base sequences in combination with steps that took into account the known biological characteristics of cell-free fetal DNA.

Fig. 2.

Detection of the fetal de novo mutations through the analysis of the sequencing data of maternal plasma DNA. The number in bold in each step represents the number of candidate de novo mutations identified after the particular process.

First, we identified genomic locations where the maternal plasma DNA sequencing showed at least one sequence variant, whereas the parental DNA analysis showed that both parents shared the same sequence. Second, we applied a dynamic cutoff algorithm to determine if the variant detected in the maternal plasma was more likely to have originated from the fetus or sequencing errors. In this algorithm, a variant would need to be observed in a threshold number of sequenced reads so as to qualify as a candidate de novo mutation ( 2018 Newest Online Free Shipping Best Wholesale Miu Miu Slingback PointedToe Pumps w/ Tags jrHo2Y
). This threshold was determined from a binomial probability function based on the total number of times that a particular nucleotide was actually sequenced and the sequencing error rate, so that the theoretical probability of a putative de novo mutation being a sequencing error would be less than 1 in 3 × 10 9 . Using this dynamic cutoff algorithm, 60,111 candidate de novo mutations were identified, and these candidate de novo mutations included 54 of 56 (i.e., 96%) de novo mutations present in the cord blood ( Fig. 2 ).

View this table:
Table S1.

Dynamic cutoff algorithm

Then, we realigned the sequenced reads covering the 60,111 candidate de novo mutation sites to the reference human genome using the BOWTIE2 software ( Bettie Page Ada Womens Unisex qWBFuCUcXZ
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, 20 ). A variant would be removed from being considered as a candidate de novo mutation if the two alignment algorithms do not map the variant to the same genomic location. This realignment procedure significantly reduced the number of false-positive results caused by alignment errors. After this realignment process, the number of candidate de novo mutations was reduced to 148 and contained 52 (93%) of the de novo mutations detected in the cord blood ( Fig. 2 ). This result is equivalent to a PPV of 35%.

8. Keen Durand Polar ($200)

In only a few years, the winter boot market has made a strong shift away from heavy pac boots like the Sorel Caribou above to more hiking-friendly models. We get the appeal—a boot like the Keen Durand Polar has a significantly more precise fit and offers nearly as much warmth and protection in most winter conditions. With solid traction in snow, a tall collar that sits partway up the shin, and decent flexibility, the Durand Polar is an excellent boot for long winter walks and snowshoe treks.

As with the standard Durand hiking boot, Keen’s Durand Polar is one of the few mainstream footwear models still made in the U.S. (the materials are sourced elsewhere, but assembly is done in Portland, Oregon). This does appear to have a slight impact on price, which runs $40 more than the Vasque Snowburban above, but we appreciate the effort nonetheless. A final fit tip: Keen recommends sizing up by half a size. Ann Demeulemeester Leather Ankle Boots Affordable Cheap Online HYUOzvASg
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9. Baffin Impact Boots ($215)

The Baffin Impact is an exercise in excess, but for truly rough and frigid conditions, it’s in a class of its own. The boot is insulated with an 8-layer system of foam and polyester, which is encased in a burly nylon and rubber shell. Winter boots are notorious for having ambitious temperature ratings (usually -25°F to -40°F), but the Impact is rated to -148°F. We haven't testedthat claim (and don't plan to), but users have reported being comfortable in the Impact in temperatures reaching as low as -50°F. As cold weather boots go, this is about as good as it gets.

The downside of so much warmth is that it doesn’t handle mild winter conditions well and isn’t very breathable. The boot also weighs nearly 6 pounds for the pair, so it’s not intended for covering much ground. But if you live in an area where the temperatures consistently dip well below zero, we heartily recommend protecting yourself with the Baffin Impact. Dearfoams Stripe ALine Slipper Womens With Paypal Online 8jooX829
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10. Steger Mukluks Yukon ($200)

Hiking and pac boots dominate the footwear options for winter, but there’s a time and place for traditional mukluks. These soft-sided boots offer fantastic insulation and comfort at a surprisingly low weight. One of the most respected designs in this category is the Yukon from Steger Mukluks. Handmade in Ely, Minnesota, the boot features a thick 9 mm wool liner, moosehide and Cordura nylon upper, and an impressive range of available sizes and widths. The look may be polarizing, but the Yukon provides Antarctic levels of warmth at about 3 pounds for a pair.

What are the downsides of the Mukluk Yukon? First off, the boot isn’t as stable or grippy on ice and snow as the more hiking-inspired designs above. The flexible construction and single strap that cinches around the ankle just can’t provide as secure of a fit as a typical lacing system. In addition, the Yukon is not waterproof and requires a treatment to keep moisture from absorbing into the leather. But in frigid conditions, nothing out there can deliver this much warmth at such a low weight. See the Steger Mukluks Yukon

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